Thayer Martin agar is a selective and differential medium used for the isolation and cultivation of Neisseria gonorrhoeae and Neisseria meningitidis, two pathogenic bacteria that cause infections in humans.
This agar was first developed by Thayer and Martin in 1964 and contains a combination of antibiotics that inhibit the growth of most bacteria while allowing the growth of Neisseria species. The antibiotics typically used in Thayer Martin agar include vancomycin, colistin, nystatin, and trimethoprim.
Thayer Martin agar is used in clinical microbiology laboratories for the diagnosis of gonorrhea and meningococcal infections. The medium is incubated in a CO2-enriched atmosphere to promote the growth of Neisseria species, which prefer microaerophilic conditions.
On Thayer Martin agar, Neisseria gonorrhoeae and Neisseria meningitidis typically appear as small, grayish colonies with a distinct morphology that helps in their identification. The differential aspect of the agar is that it allows for the differentiation of these species from other bacteria that may also grow on the medium.
Overall, Thayer Martin agar is an important tool in the isolation and identification of Neisseria species and plays a crucial role in the diagnosis and management of infections caused by these pathogens.
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